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. 2010 Feb 11;285(15):11652–11666. doi: 10.1074/jbc.M109.051094

FIGURE 9.

FIGURE 9.

A specific interaction between Gas7 and N-WASP regulates the neurite outgrowth of hippocampal neurons. A, immunostaining of cultured hippocampal neurons transfected with EGFP, WW2/EGFP, or EGFP/Pro is shown. Cultured hippocampal neurons were transfected with EGFP, WW2/EGFP, or EGFP/Pro at DIV2 and immunostained at DIV4. Neurite outgrowth was aberrant in WW2/EGFP and EGFP/Pro-overexpressing cultured hippocampal neurons as compared with control EGFP-overexpressing cells. Scale bar, 20 μm. B, shown is quantification of morphological changes in neurite outgrowth from A. The ratio of longest neurite length to cell body diameter decreased significantly with mutant transfection (WW2-EGFP, n = 27; EGFP-Pro, n = 25; control EGFP, n = 32; unpaired Student's t test; **, p < 0.01; ***, p < 0.001).