Table 1.

Primers Used in Real-Time PCR Experiments

Primers	Sequence	Annealing temperature (°C)
GAPDH	F: GACAGGATGCAGAAGAGATTACT R: TGATCCACATCTGCTGGAAGGT	55
γ-GCS	F: AACACAGACCCAACCCAGAG R: GTCTGGCTGAGAAGCCTTTG	59
GST	F: TACTTTGATGGCAGGGGAAG R: TCATCCCGTCGATCTCTACC	58
NQO1	F: TTCTTCTGGCCGATTCAGAGT R: TCCAGACGTTTCTTCCATCC	55
Nrf2	F: GGGGACAGAATCACCATTTG R: GATGCAGGCTGACATTCTGA	57
PON2	F: ATGGTGGCTCTGAGTTTGCT R: TCCTCAGCTCCAGTTTCGAT	57
SIRT1	F: GTCTCCTGTGGGATTCCTGA R: ACACAGAGACGGCTGGAACT	57

GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; γ-GCS, γ-glutamyl cysteine synthetase; GST, glutathione-S-transferase; NQO1, nicotinamide adenine dinucleotide phosphate [NAD(P)H]:quinone oxidoreductase; PON2, paraoxonase-2; F, forward; R, reverse.