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Analysis of in vitro generated RNA transcripts by agarose gel electrophoresis. NotI linearized pMD19-CV was transcribed with or without T7 RNA polymerase. The resultant reaction mixtures were analyzed by electrophoresis on a 1.2% agarose gel. Lane M, ssRNA ladder marker (Cat#N0362S, New England Biolabs); lane 1, reaction mixture with T7 RNA polymerase; lane 2, reaction mixture without T7 RNA polymerase.
