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Western blot analysis of capsid composition of the recovered viruses. Lysates from cells infected with the R1 or R2 generation of recovered viruses or wild-type virus, were separated by SDS-PAGE, blotted onto PVDF membranes, and probed with polyclonal anti-CVVP0, anti-CVVP1, or anti-CVVP3, followed by incubation with an HRP-conjugated secondary antibody.
