Table 1.
Primers used in this study
| Primer | Sequence (5' - 3') | Enzyme site | Purpose |
|---|---|---|---|
| P1 | GCCAAGCTTAAAACAGCCTGTGGGTTGTTCCCACCC | Hind III | CV(1-4392) amplification |
| P2 | CGGGTCTAGAGCGTAGACTCTTTTGGCTTCAGTC | Xba I | CV(1-4392) amplification |
| P3 | CTACGCTCTAGAAAGAAGGA | Xba I | CV(4381-7410) amplification |
| P4 | ACAAGCGGCCGCTGCTATTCTGGTTATAAC | Not I | CV(4381-7410) amplification |
| P5 | CTTCTCGAGGTTGATTTTGAGCAAGCATTG | Xho I | CV(6087-7410-pA) amplification |
| P6 | TATGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTT | Not I | CV(6087-7410-pA) amplification |
| P7 | CTAAAGCTTAGCTAATACGACTCACTATAGTTAAAACAGCCTGTGGGTTG | Hind III | T7 promoter introduction/priming cDNA synthesis from negative-strand RNA |
| P8 | CCTATTGCAGACATGATTGACCAG | none | RT-PCR for negative-strand RNA |
| P9 | TGTTGTTATCTTGTCTCTACTAGTG | none | RT-PCR for negative-strand RNA |
Restriction enzyme sites are underlined