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. 2012 Feb 21;6(2):e1526. doi: 10.1371/journal.pntd.0001526

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Zornetta et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Time course of Cyt c release from isolated tibialis anterior mice muscles in the incubation medium after addition of BaV (A) or CdV (B). The protein concentrations were determined and 2.5 µg of total proteins were loaded in each lane. Western blots depicting the time course of Cyt c release (A) after BaV treatment (50 µg/ml) or (B) after CdV (50 ug/ml) and the same volume of vehicle as control. (C) The graphs report the quantitative analysis of the kinetics of Cyt c release induced by venoms (black lines) and controls (dotted lines). The intensity of each band was determined using the software Quantity One (Bio-Rad) The blots and their quantification show one representative experiment (n≥3).