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. 2012 Feb 21;7(2):e30960. doi: 10.1371/journal.pone.0030960

Figure 1. Virus antigen expression in tissues of infected chickens.

Figure 1

Chickens were infected with HP VN/1203/E (A, C, E) and LP VN/1203/E (B, D, F). Tissues were taken at 18–36 hours pi from HP infected birds and at 5–13 days pi from LP infected birds. Sections were prepared from brain (A, B), skin from the comb (B, C) and heart (E, F) and stained for the presence of viral antigen. A. Brain, showing high levels of antigen in capillaries (arrows) and in occasional neurons. B. Brain, 13 days pi, showing antigen in neurons within a focus of glial proliferation. C. Skin of comb, showing antigen in capillaries (arrows) and surrounding connective tissue within the dermis. D. Skin of comb, 13 days pi, showing viral antigen in the stratum granulosum. E. Heart, showing antigen predominantly in capillaries (arrows) and in the myocardium (indicated by arrow heads). F. Heart, 5 days pi, showing viral antigen in a single myocardial fiber. All scale bars are 100 µm.