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. 2012 Feb 21;7(2):e31296. doi: 10.1371/journal.pone.0031296

Figure 1. In vitro-generated Th1 and Th17, but not Th2 or iTreg cells mobilize pCD40L.

Figure 1

A and B, Mobilization assay. In vitro-generated Th1, Th2, Th17, and iTreg cells were stimulated with PMA plus ionomycin or left unstimulated in the presence of PE-isotype Ab, PE-anti-CD40L or PE-anti-CTLA-4 at 37°C for 30 minutes. The levels of CD40L (A) and CTLA-4 (B) are shown. C and D, Intracellular staining. Cells were fixed without stimulation, permeabilized, and stained with PE-isotype Ab, PE-anti-CD40L or PE-anti-CTLA-4. The levels of CD40L (C) and CTLA-4 (D) are shown. Data are representative of five independent experiments.