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. 2012 Feb 21;7(2):e31296. doi: 10.1371/journal.pone.0031296

Figure 4. Excess IL-4 downregulates pCD40L levels in Th2 cells generated in vitro.

Figure 4

Recovery of pCD40L in Th2 cells by neutralizing IL-4. A–C, Differentiated Th2 cells from 4get/DO11.10 spleen cells were restimulated with peptide-pulsed splenic APCs alone (Th2 2°) or in the presence of exogenous IL-4 (Th2 2° plus IL-4) or anti-IL-4 (Th2 2° plus anti-IL-4) for 4 days. A, The levels of IL-4/eGFP in each group of Th2 cells as well as Th1 negative control cells. B, IL-4 production upon PMA plus ionomycin stimulation by each group of Th2 cells was analyzed by ELISA. Each bar represents the mean ± standard deviation for triplicates. C, Mobilization of pCD40L by each group of Th2 cells in response to PMA plus ionomycin. Data are representative of two independent experiments.