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. 2012 Feb 21;7(2):e31296. doi: 10.1371/journal.pone.0031296

Figure 5. In vivo-generated Th17 and Th17/Th1 cells store and mobilize pCD40L.

Figure 5

A, CNS infiltrating leukocytes were obtained from brains and spinal cords of EAE animals induced by immunization with CFA plus MOG peptide and pertussis toxin. B, CNS infiltrating leukocytes were analyzed by the CD40L mobilization assay followed by intracellular cytokine staining. C, Intracellular staining of IL-17A and IFN-γ in CNS CD4+ T cells. D, CD40L mobilization in Th1 (IL-17A−IFN-γ+), Th17 (IL-17A+IFN-γ−), Th17/Th1 (IL-17A+IFN-γ+), and antigen non-specific (IL-17A−IFN-γ−) CD4+ T cells upon antigenic stimulation. Data are representative of three independent experiments.