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. 2012 Feb 21;7(2):e31324. doi: 10.1371/journal.pone.0031324

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The C-terminal Yc fusion of each of the proteins was co-expressed with TMD-Yn (1^st column) or Yn-TMD (2^nd column) and the C-terminal Yn fusion of each of the protein was co-expressed with TMD-Yc (3rd column) or Yc-TMD (4^th column). The fluorescence complementation with the lumenal reporters and the lack of same with the cytosolic reporters indicate Golgi lumenal orientation of the IRX10L, RGXT2 and UXS2 catalytic domains. Scale bars, 10 µm. At least two individual experiments were performed for each combination with the similar results. Raw images are shown.