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. 2012 Feb 21;7(2):e31324. doi: 10.1371/journal.pone.0031324

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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ARAD1 and MUR4 fused with the Yc and Yn tags, respectively, at the C-termini were transiently expressed in Nicotiana benthamiana. Fluorescence complementation with lumenal reporters suggests Golgi-lumenal localization of ARAD1 and MUR4 C-termini. Observation was carried out by CLSM at 3 DPI. Scale bars, 10 µm. At least two individual experiments were performed for each combination with the similar results. Raw images are shown.