Figure 1. Mutation in barA or uvrY reduces virulence in murine ascending UTI.

CBA/J mice were infected transurethrally with mixture of 5×10⁷ CFU of WT bacteria and 5×10⁷ CFU of mutant bacteria. A. Bacteria were recovered 72 hr later from mice infected with CFT073 WT urine (○), SM3009 (barA::cm) urine (•), CFT073 WT bladder (Δ), SM3009 (barA::cm) bladder (▴), CFT073 WT kidneys (□), SM3009 (barA::cm) kidneys (▪) and were expressed in Log₁₀ CFU. B. Bacteria were recovered 72 hr later from mice infected with CFT073 WT urine (○), SM3010 (uvrY::cm) urine (•), CFT073 WT bladder (Δ), SM3010 (uvrY::cm) bladder (▴), CFT073 WT kidneys (□), SM3010 (uvrY::cm) kidneys (▪) and were expressed in Log₁₀ CFU. The results were representative of two independent experiments. Star denotes P<0.01.