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. 2012 Feb 21;7(2):e31348. doi: 10.1371/journal.pone.0031348

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A set of twelve 12-day-old SPF embryonated eggs were inoculated through allantoic cavity with 5×10³ CFU of bacteria CFT073 WT (▪), DH5αΚ12 (□), SM3009 (barA::cm) (▴), SM3012 (barA/p-barA) carrying pSM1 (Δ), SM3010 (uvrY::cm) (•), SM3013 (uvrY/p-uvrY) carrying pSM2 (○), SM3011 (csrA::cm) (♦) and SM3014 (csrA/p-csrA) carrying pSM7 (X). The results were scored as live, morbid or dead. The results were representative of two independent experiments.