Table 3. Mutation in uvrY reduces invasion of E. coli CFT073 strain to ureter (SV-HUC-1) uroepithelial cells.
| Genotype | Initial cells (log10 CFU /ml) | Attached and invaded after 2 h (log10 CFU / ml) | Invaded fraction surviving after 4 h (log 10 CFU / ml) | Calculated attached bacteria (log 10 CFU/ml) | Attachment Index | Invasion Index |
| CFT073 WT | 8.4±0.9 | 7.4±0.8 | 3.1±0.5 | 7.5±0.8 | 7.7×10−2 | 6.8×10−5 |
| barA | 8.3±0.9 | 7.2±0.9 | 3.0±0.5 | 7.2±0.9 | 8.3×10−2 | 6.6×10−5 |
| barA/p-barA | 8.9±0.9 | 7.7±0.9 | 3.3±0.5 | 7.7±0.9 | 6.3×10−2 | 4.7×10−5 |
| uvrY ** | 8.6±0.9 | 6.8±0.8 | 0.7±0.1 | 6.8±0.8 | 1.7×10−2 | 7.1×10−7 |
| uvrY/p-uvrY | 8.8±0.9 | 7.6±0.9 | 2.9±0.5 | 7.6±0.9 | 6.3×10−2 | 1.9×10−5 |
| csrA | 7.7±0.9 | 6.6±0.8 | 2.2±0.3 | 6.6±0.8 | 8.9×10−2 | 3.6×10−5 |
| csrA/p-csrA | 7.7±0.9 | 6.6±0.8 | 0.7±0.1 | 6.6±0.7 | 7.8×10−2 | 1.3×10−6 |
Results represent mean ± SD from three individual experiments.
**Mutation in uvrY significantly reduces the virulence (p≤0.01, relative to WT).