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. 2012 Feb 21;7(2):e31788. doi: 10.1371/journal.pone.0031788

Figure 8. RT-PCR analysis of wild-type M. smegmatis and Myco132 mRNA.

Figure 8

mRNA was isolated from intracellular and extracellular bacteria and amplified by RT-PCR. Products were resolved on a 1% agarose gel. Lane M, TrackIt™ 100 bp DNA ladder (Invitrogen). Lanes 1, 3, 5, 7: intracellular WT, extracellular WT, intracellular Myco132 and extracellular Myco132, respectively. Lanes 2, 4, 6, 8: RT minus controls for intracellular WT, extracellular WT, intracellular Myco13 and extracellular Myco132, respectively. Lane 9: gDNA positve control. Lane 10: negative PCR control.