Table 4. Apple Infinium® II array v1 content and evaluation in the Plant & Food Research dataset. The number of attempted SNPs and clusters is indicated per linkage group (LG), as well as their density based on the apple genome assembly in kilobases (Kb) and the ‘Golden Delicious’ genetic map (cM).
Attempted SNPs on IRSC Apple Infinium II array v1 | Array evaluation in the Plant & Food Research dataset | ||||||||||
LG | No. of SNPs | No. of clusters | Average physical distance between focal points (kb) | Average genetic distance between focal points (cM) | No. of successful beadtypes | No. of poly-morphic SNPs | % poly-morphic SNPs | No. of poly-morphic SNPs with MAF>0.05 | MAF>0.05, 50 GC>0.4, call rate >0.95 | No. of poly-morphic clusters | % poly-morphic clusters |
1 | 443 | 81 | 443.7 | 1.10 | 434 | 299 | 68.9 | 250 | 180 | 75 | 92.6 |
2 | 695 | 113 | 355.1 | 0.75 | 684 | 519 | 75.9 | 436 | 313 | 108 | 95.6 |
3 | 499 | 74 | 538.3 | 1.19 | 487 | 350 | 71.9 | 288 | 212 | 72 | 97.3 |
4 | 392 | 65 | 388.7 | 1.00 | 386 | 292 | 75.6 | 235 | 201 | 63 | 96.9 |
5 | 492 | 82 | 456.9 | 1.10 | 486 | 360 | 74.1 | 308 | 239 | 76 | 92.7 |
6 | 351 | 64 | 473.2 | 1.17 | 340 | 233 | 68.5 | 194 | 117 | 63 | 98.4 |
7 | 345 | 71 | 438.5 | 0.83 | 340 | 215 | 63.2 | 181 | 129 | 62 | 87.3 |
8 | 405 | 68 | 519.9 | 1.07 | 399 | 276 | 69.2 | 229 | 182 | 62 | 91.2 |
9 | 490 | 80 | 449.2 | 0.91 | 477 | 364 | 76.3 | 311 | 232 | 76 | 95.0 |
10 | 554 | 83 | 446.6 | 1.12 | 531 | 377 | 71.0 | 271 | 224 | 79 | 95.2 |
11 | 466 | 80 | 500.7 | 0.97 | 456 | 322 | 70.6 | 277 | 177 | 73 | 91.3 |
12 | 469 | 85 | 426.5 | 0.87 | 459 | 338 | 73.6 | 258 | 210 | 81 | 95.3 |
13 | 433 | 81 | 489.6 | 0.94 | 423 | 299 | 70.7 | 229 | 178 | 76 | 93.8 |
14 | 388 | 72 | 472.2 | 1.02 | 374 | 266 | 71.1 | 215 | 151 | 64 | 88.9 |
15 | 629 | 106 | 525.6 | 1.09 | 621 | 440 | 70.9 | 365 | 253 | 101 | 95.3 |
16 | 355 | 74 | 316.2 | 0.76 | 347 | 271 | 78.1 | 215 | 158 | 69 | 93.2 |
17 | 461 | 76 | 356.7 | 0.99 | 448 | 333 | 74.3 | 231 | 215 | 70 | 92.1 |
Total | 7867 | 1355 | 446.2 | 1.00 | 7692 | 5554 | 72.2 | 4493 | 3371 | 1270 | 93.7 |
The Apple Infinium® array was evaluated by screening 1398 progeny in 8 segregating populations and 117 individuals from the Plant & Food Research apple germplasm collection.