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. 2012 Feb 21;7(2):e31797. doi: 10.1371/journal.pone.0031797

Figure 2. SDS-PAGE electrophoresis analysis of WT and cysteine mutants of Viperin.

Figure 2

Sample lanes are as follows: (1) supernatant of WT Viperin (2) inclusion body fraction of WT Viperin (3) supernatant of C90A mutant (4) inclusion body fraction of C90A mutant (5) supernatant of C87A mutant (6) inclusion body fraction of C87A mutant (7) supernatant of C83A mutant (8) inclusion body fraction of C83A mutant (9) supernatant of the triple cysteine mutant (10) inclusion body fraction of the triple cysteine mutant (11) a molecular weight ladder. The WT protein has been found to express well in the supernatant. In contrast, all the cysteine mutants are expressed as inclusion body.