Table 1. The hydrodynamic radius (RH) of the proteins calculated from DLS, Native gel, SEC and SAXS experiments.
Protein | Calculated RH (Å) | |||
DLS | Native Gel | Gel Filtration | SAXS † | |
hPPARγ LBD monomer | 27.5 | N/C* | 28.0 | 27.4 |
hPPARγ-hRXRα LBD | 39.0 | 39.0 | 39.0 | 36.7 |
hRXRα LBD dimer | 38.0 | 38.0 | 36.0 | ----------- |
hRXRα LBD tetramer | 43.0 | 43.0 | 42.0 | ----------- |
RH calculated from Rg (Guinier analysis) using the relation between them: RH = Rg×1.3.