Table 2. Structural Parameters Derived from SAXS for hPPARγ LBD (monomer) and hPPARγ/hRXRα LBD (heterodimer).
| Parameters | Monomerexp† | Monomerhigh-resolution model ‡ | MonomerDAM € | Heterodimerexp† | Heterodimerhigh-resolution model‡ | HeterodimerDAM € | HeterodimerRigid body model <$>\raster="rg2"<$> |
| Dmax (Å) | 65.0±5.0 | 64.2 | 62 | 85.0±5.0 | 85.4 | 85.7 | 89.1 |
| Rg (Å) | 21.0±0.1 | 20.2 | 20.1 | 28.2±0.1 | 25.5 | 27.9 | 26.4 |
| (Gnom) | (Gnom) | ||||||
| 21.1 | (Crysol) | (Crysol) | 29.1 | (Crysol) | (Crysol) | (Crysol) | |
| Guinier | (Guinier) | ||||||
| Resolution (Å) | ------------ | ------------ | 25.4 | ------------ | ------------ | 25.6 | --------------- |
| MWSAXS (kDa) § | 31.1 | ------------ | ------------ | 65.1 | ------------ | ------------ | ------------ |
| MWBSA (kDa) & | 30.9 | ------------ | ------------ | 57.9 | ------------ | ------------ | ------------ |
| MWSAXS MoW (kDa) # | 33.0 | ------------ | ------------ | 73.9 | ------------ | ------------ | ------------ |
| MWtheoretical (kDa) | 31.3 | ------------ | ------------ | 57.8 | ------------ | ------------ | ------------ |
†
Calculated from the experimental data.
‡
Values of hPPARγ LBD monomer and hPPARγ/hRXRα LBD heterodimer from the crystallographic model data (PDB id 1FM6).
€
Parameters of the Dummy Atom Models.
<$>\raster="rg1"<$>Parameters of Rigid Body Model. Resolution: 2π/qmax.
§
Experimental estimate of the Molecular Weight (MW) using the forward scattering I(0)/c at the absolute scale using water as a standard [21].
&
MW computed from the scattering data using BSA [22] as a secondary standard.
#
Estimate of the MW using SAXS MoW [23].