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. 2012 Feb 21;7(2):e31854. doi: 10.1371/journal.pone.0031854

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Suzuki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic of the targeting strategy used for disruption of the first coding exon of CALM. (B) Southern hybridization analysis of DNA isolated from targeted mouse embryos. From left to right: 5′ probe, 3′ probe and neo probe, as shown in A. (C) Genomic PCR analysis of DNA isolated from mouse tail. The genotypes are indicated above each lane. (D) Immunoblot analysis of stable MEF cell lines from wild-type (+/+) and CALM-deficient (−/−) mouse embryos (E14.5). The expected molecular weights of CALM are 62 and 72 kDa (arrows). Actin was used as a loading control.