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. 2012 Feb 21;7(2):e31900. doi: 10.1371/journal.pone.0031900

Figure 4. Immunohistochemistry (IHC) analysis of therapeutic effects.

Figure 4

Tsc2+/− A/J strain mice of ages 9–10 months were treated with drugs for 1 week, and kidneys prepared for histology. Sections were prepared and stained using pS6-S235/236 (red), pACC-S79 (red), pIκBα-S32/34 (red) and GRP78 (red) antibodies. The bottom panel shows apoptosis assessed by TUNEL method. Representative sections are shown. All images shown are taken at 100× magnification. Insets show portions of the tumor at higher magnification (400×).