Figure 7. Conservation of putative active sites in three pilY1 homologs in V. paradoxus EPS.

ClustalW alignment of PilY1 around (A) the N-terminal conserved site previously described in [29] and (B) the C-terminal Ca²⁺-binding site [27]. Varpa_5900 and its ortholog Vapar_5179 in V. paradoxus S110 are outlined in red, and the conserved functional sites in each region are outlined in black. Ne = Nitrosomonas europaea ATCC 19718 NE_1747,Thio = Thioalkalivibrio sulfidophilus HL-EbGr7 Tgr7_3225, Pm ymp = Pseudomonas mendocina ymp Pmen_0958, PAO1 = Pseudomonas aeruginosa PAO1 PA4554, PAK = Pseudomonas aeruginosa PAK PilY1.