Table 2. Strains, Vectors, Primers.

Name	Description	Source/other info
Bacterial Strains
Variovorax paradoxus EPS	Wild type strain collected at CSUSB	this study
V. paradoxus EPS mut86	Tn5 insertion in wbuB at nt24	this study
V. paradoxus EPS mut223	Tn5 insertion in pilY1 at nt1347	this study
V. paradoxus EPS mut99	Tn5 insertion in shkS at nt413	this study
E. coli DH5α	F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK − mK +), λ–	[40]
E. coli S17-1λpir	thi pro hsdR − hsdM+ recA RP4 2-Tc::Mu-Km::Tn7(TpR SmR) λ-pir	[41]
Plasmids
pOT182	pSUP102::Tn5	[33]
pCR2.1	TA vector	Invitrogen
pBBR1MCS-2	Broad host range vector	[42]
pWbuB	pBBR1MCS2:Varpa_4680	This study
pPilY1	pBBR1MCS2:Varpa_5900	This study
pShkR	pBBR1MCS2:Varpa_4664	This study
pShkS	pBBR1MCS2:Varpa_4665	This study
Primers
Erc2	ACAGATTTAGCCCAGTCGG	Sequencing rescue clones
Hgal	GTCAGCACCGTTTCTG
Wbub n-term	CCATGGGTCTTTGGAGATGATTATCCA	NcoI at 5′ end
Wbub c-term	GAGCTCCCCCAATAGAACTGAGAAAT	SacI at 5′ end
PilY1 n-term	AAATCCCATTTGAACCAGAG
PilY1 c-term	GTTAACGACTGGACAGAGAAAAGG
shkR forward	GAATTCCGCTGGAGGCATTGGCGGAA	EcoRI at 5′ end
shkR reverse	ACTAGTGACGAGGCGGTGGCACAGG	SpeI at 5′ end
shkS forward	GAATTCGCCGGTGCCGTCGAACTTGA	EcoRI at 5′ end
shkS reverse	ACTAGTCTGCACTACGCCCGCACCAA	SpeI at 5′ end
Lucfor	TACAACACCCCAACATCTTCGA	[22]
Lucrev	GGAAGTTCACCGGCGTCAT	[22]
Wbub for	GGCGTCTCTGTGGCCTGCG
Wbub rev	GCGGCCAAATATACGCGCACC
PilY1 for	GCAGCTCGAGCCTTGGCAACT
PilY1 rev	CGCCCCTGTATAGCGCCGTG