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. 2012 Feb 21;7(2):e31978. doi: 10.1371/journal.pone.0031978

Figure 3. Margatoxin increases mitral cell excitability.

Figure 3

(A) Representative action potential trains in a mitral cell in response to a 75 pA current injection of 1000 ms duration before (left) and approximately 10 min after 1 nM Margatoxin (MgTX) bath application (right). (B) Representative action potential train in a mitral cell in response to a 100 pA current injection of 1000 ms duration before (left) and after after MgTX (center) and then after subsequent 10 ng/ml BDNF bath addition (right). The resting membrane potential was held near −65 mV. (C) Line graph of the mean (± s.e.m.) spike frequency evoked at each current injection as recorded in A and B. Closed black circles represent data before BDNF stimulation (Before) and open red circles represent the same neurons after MgTX stimulation (MgTX). Open red squares represent the subsequent application of BDNF while maintaining MgTX block (MgTX+BDNF) as in B. (D) Line graph of the mean (± s.e.m.) ISI at each current injection. Data were fit with linear regressions to facilitate visualization. MgTX has a significant treatment effect for both parameters; two-way ANOVA (N = 5; p≤0.05). BDNF application following MgTX did not significantly alter either rate or ISI; two-way ANOVA (N = 3; p≥0.05).