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. 2012 Feb 21;7(2):e32191. doi: 10.1371/journal.pone.0032191

Figure 6. IGFs induce endothelial differentiation at early and late stages of vasculogenesis and increase expression of VEGF.

Figure 6

A–B) VEGFR2 and Tie-2 levels at day 7 are significantly increased in EB treated on days 1 and 2 only or on days 4–7 only. C) Treatment with IGF1 or IGF2 post-mesoderm development results in an increase in the percentage of VWF-positive cells compared to control differentiation EB. D–E) PPP treatment on days 1 and 2 only or on days 4–7 only reduced levels of endothelial markers at day 7. Levels of VEGF increased with IGF1 and IGF2 treatment, and decreased with PPP treatment (F–H), at day 3. * denotes P<0.05 compared to control ** P<0.01, Rn denotes normalized reporter.