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. 2012 Feb 21;7(2):e32102. doi: 10.1371/journal.pone.0032102

Figure 4. The effect of HIV-1 on cytokine mRNA levels of MDM exposed to antibody opsonised IE.

Figure 4

MDM from 6 donors cultured in 24-well plates were infected with HIV-1Ba-L (•) or mock-infected (○) and primed for 48 hrs with IFNγ then exposed to IE opsonised with 9% pooled immune serum (IE-PPS) at a target to cell ratio of 20∶1 for 2 hrs followed by preparation of RNA and cDNA for analysis of (A) IL-1β, (B) TNF and (C) IL-6 cytokine mRNA levels by qPCR. Comparisons of mRNA levels between cultures infected with HIV-1Ba-L or mock-infected exposed to IE-PPS were made using the Mann-Whitney non-parametric U test.