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. 2011 Nov 3;82(6):1375–1391. doi: 10.1111/j.1365-2958.2011.07892.x

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© 2011 Blackwell Publishing Ltd

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Fig. 2 — Analysis of NudC native structure.

A. Nickel affinity-purified NudC_BCG (lane 1, arrow) and NudC_Rv (lane 2, arrow) were electrophoresed on 10% (v/v) SDS-PAGE; lane 3: protein molecular weight marker.

B. Native PAGE of purified NudC_BCG (lane 1) and NudC_Rv (lane 2) on 9% (v/v) PAGE. Two NudC bands were detected and are indicated as α₂ and α (arrows indicated).

C. Gel exclusion chromatography analysis of the native structure of purified NudC_Rv and NudC_BCG. Nickel affinity-purified NudC_BCG and NudC_Rv were concentrated then loaded onto a Superdex 200 10/300GL column and eluted.

D and E. Analytical ultracentrifugation analysis of the native structure of purified NudC_BCG (D) and NudC_Rv (E). Expected products are indicated by arrows.