Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 3;82(6):1375–1391. doi: 10.1111/j.1365-2958.2011.07892.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Blackwell Publishing Ltd

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

PMC Copyright notice

Fig. 5 — Inhibition of mycobacterial mycolic acid biosynthesis by INH and ETH. M. smegmatis mc²155, mc²155 ΔnudC and mc²155 pMV261::nudC_BCG were treated with serial concentrations of INH (A) or ETH (B) for 4 h, and then labelled with 1, 2-[¹⁴C] acetate for another 4 h. FAMEs and MAMEs were then extracted and separated by TLC. ¹⁴C-labelled FAMEs and MAMEs were detected by autoradiography after overnight exposure to a Kodak BioMax MR film. Similarly, M. bovis BCG, BCG ΔnudC and BCG pMV261::nudC_BCG were treated with serial concentrations of INH (C) or ETH (D) for 24 h. Then 1, 2-[¹⁴C] acetate was added and cultures were further incubated for another 24 h. FAMEs and MAMEs were then extracted and separated by TLC. ¹⁴C-labelled FAMEs and MAMEs were detected by autoradiography after overnight exposure to a Kodak BioMax MR film.