Fig. 3.

Electrophoretic separation of total RNA isolated from the leaves of S. viminalis in 1.2% denaturating gel electrophoresis with formaldehyde, stained with etidium bromide. Lanes: 1 Ctr, 2 Hm, 3 B1, 4 B2, 5 B3, 6 B4, 7 Hm + B1, 8 Hm + B2, 9 Hm + B3, 10 Hm + B4, 11 negative Ctr (noninoculated plants growing at noncontaminated soil) (a). Hybridization northern of molecular probe MT1-labeled with digoxygenin to RNA isolated from the leaves of S. viminalis clone 87–101 growing in the pot experiment in heavy metal-contaminated soil. Description of lanes as in a (b). Differential expression of MT1 gene in various variants of inoculation. Description of columns as in a (c)