Tracking of single NPs, transported in living D. discoideum cells, yields this type of trajectories. Cytoskeleton depolymerization agents, namely Benomyl and Latrunculin A, are used to discern the influence of particular cytoskeleton constituents, MT and F-actin, on the intracellular transport. Trajectories are analyzed using a local MSD algorithm shifting a rolling window across the NP trajectories.