Figure 5.

EGFP aggregation as a function of [TFE]. The plot shows the scattering signal from 50 μM EGFP at pH 2.4 (solid bars), pH 7.5 (dark-shaded bars), and pH 2.4 with 75 mM NaCl (light-shaded bars). The samples were incubated for 24 ± 2 h at 37°C in the presence of 0, 7.5, 15, 22.5, 30, 45, and 60% TFE. (Error bars) Standard deviations of measurements of three identical samples and baseline uncertainties.