Table 1.

Relative free energies of membrane insertion for OmpLA mutants

Residue	WTB scale (kcal/mol)	Aqueous reference state
		Folded	Seven-residue strand	Isolated
$\Delta \Delta G_{\text{aq}.\to \text{DLPC}}^{\text{OmpLA}}$ (Arg)	3.71 ± 0.13	3.76 ± 0.27	1.06 ± 0.22	2.65 ± 0.20
$\Delta \Delta G_{\text{aq}.\to \text{DLPC}}^{\text{OmpLA}}$ (Leu)	−1.81 ± 0.13	−0.91 ± 0.06	−2.64 ± 0.08	−2.06 ± 0.07
$\Delta \Delta G_{\text{aq}.\to \text{DLPC}}^{\text{OmpLA}}$ (Ser)	1.83 ± 0.22	4.08 ± 0.05	3.52 ± 0.06	5.24 ± 0.06