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. 2012 Jan 15;125(2):473–485. doi: 10.1242/jcs.093344

Fig. 3.

Fig. 3.

Decrease in [Na+]i occurs after activation of PKA and before the increase in tyrosine phosphorylation. Sperm from the cauda epididymus were loaded with CoroNa Red, as described, for 30 minutes, then washed and incubated for different time periods in medium that supported capacitation. (A–D) Two-dimensional fluorescence dots plots of CoroNaRed versus PI of sperm incubated for 0 (A), 10 (B), 30 (C) and 60 (D) minutes. (E) CoroNa Red fluorescence in the live sperm population at each time point. Values are means ± s.e.m. (n=5, *P≤0.05 and **P≤0.01). (F) Western blots, using anti-PKAS-P (left panel) and anti-Y-P (right panel), of sperm extracts incubated for different time periods in the same media that were used for flow cytometry experiments.