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. 2011 Nov 30;21(6):1299–1311. doi: 10.1093/hmg/ddr559

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© The Author 2011. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — VAPB is a MAM protein. (A) MAM, mitochondria (Mito) and ER fractions were prepared from HEK293 cells and equal protein amounts from each fraction probed on immunoblots for PTPIP51 and HSP60 (mitochondrial proteins) FACL4 (MAM enriched protein), PDI (ER protein) and VAPB. (B) A proportion of VAPB colocalizes with IP3R3 and mitochondria. CV1 cells were immunostained for VAPB, IP3R3 (MAM enriched protein) and HSP60 (mitochondrial protein). Some punctate VAPB and IP3R3 staining aligned with mitochondria (Merge). Zoomed cellular areas are indicated [zoom (a), and zoom (b)]. Colocalized VAPB and IP3R3 labelling along mitochondria is indicated with arrowheads. Scale bar, 20 μm; zoom (a), 4 μm.

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