Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 30;21(6):1299–1311. doi: 10.1093/hmg/ddr559

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2011. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Compared to VAPB, VAPBP56S levels associated with PTPIP51 are elevated and VAPBP56S levels are increased in MAM. (A) VAPBP56S binds more PTPIP51 than VAPB in immunoprecipitation assays. HEK293 cells were co-transfected with either HA-PTPIP51 and myc-VAPB, or HA-PTPIP51 and myc-VAPBP56S. myc-VAPB/VAPBP56S or HA-PTPIP51 were immunoprecipitated using anti-myc or anti-HA antibodies and bound HA-PTPIP51/myc-VAPB then detected on immunoblots. Control immunoprecipitations (Ctrl) were performed with non-immune antibody. Upper panel shows myc-VAPB/VAPBP56S immunoprecipitations; middle panel shows HA-PTPIP51 immunoprecipitations. Lower panel shows input levels of transfected proteins. Bar chart shows relative amounts of co-immunoprecipitated PTPIP51 (upper) and VAPB (middle) following densitometric quantification of signals. **P < 0.01, n = 3, t-test. Error bars are mean ± SEM. (B) Compared to VAPB, VAPBP56S levels associated with mitochondria are increased. Mitochondria containing MAM were purified from HEK293 cells transfected with empty vector (Ctrl), myc-VAPB (VAPB) or myc-VAPBP56S (P56S). The samples were then probed on immunoblots for myc-VAPB/VAPBP56S using anti-myc antibody, tubulin and COXIV (as a mitochondrial marker). Bar chart shows relative amounts of myc-VAPB and myc-VAPBP56S following densitometric quantification of signals. *P< 0.05, n = 3, t-test. Error bars are mean ± SEM. (C) Compared to VAPB, VAPBP56S levels are increased in MAM and decreased in non-MAM ER. HEK293 cells transfected as in (B) were fractionated into MAM, pure mitochondria and ER. The samples were then probed on immunoblots for myc-VAPB, myc-VAPBP56S, IP3R3 (MAM marker), COXIV (mitochondria marker) and PDI (ER marker). Total shows immunoblot of the total cell lysates (before fractionation); equal loading was confirmed by immunoblot for tubulin.

HHS Vulnerability Disclosure