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. 2011 Dec 2;21(6):1312–1324. doi: 10.1093/hmg/ddr568

Figure 3.

Figure 3.

Mbnl1 knockdown enhances CaV1.1 E29 skipping in C2C12 mouse myoblasts. (A) Immunoblot for Mbnl1 in C2C12 myoblasts treated with mock transfection (lane 1), si-RNA targeting Mbnl1 (lanes 2 and 3) and scrambled si-RNA control (si-ctrl). Efficient knockdown was achieved with si-Mbnl1-No.5 but not si-Mbnl1-No.8. RT–PCR assay for alternative splicing of CaV1.1-E29 (B), Cypher exon 11 (C) and Serca1-E22 (D) (same cells/lanes as labeled in A and C). Quantification of CaV1.1-E29 inclusion for each condition is shown in the lower panel of (B). Mbnl1-knockdown significantly reduced CaV1.1-E29 inclusion (**P < 0.01).