Figure 5.
Overexpression of CUGBP1 represses CaV1.1-E29 in mouse TA muscles. (A) Immunoblot for the CUGBP1 protein in mouse TA 9 days after intramuscular injection and electroporation of CUGBP1 expression construct (lanes 1 and 3) or empty vector control (lanes 2 and 4). GAPDH was used as a loading control. (B) RT–PCR assay of CaV1.1 E29 (upper), Serca1-E22 (middle) and Capzb—exon 8 (lower) splicing in mouse TA muscle 9 days after injection/electroporation of CUGBP1 expression construct (lanes 1 and 3) or empty vector control (lanes 2 and 4).
