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. 2011 Dec 2;21(6):1312–1324. doi: 10.1093/hmg/ddr568

Figure 8.

Figure 8.

CaV1.1-E29 skipping in TA of HSALR mice increases the percentage of fibers with central nuclei. (A) RT–PCR analysis of CaV1.1-E29 (upper) and Serca1-E22 (lower) splicing in TA muscles of HSALR mice, 3 months following a single injection and electroporation of ΔE29 (T) or control (C) morpholinos. The left TA (LTA) or right TA (RTA) muscles of the same mouse were randomly selected for the treatment with either control or ΔE29 morpholinos. (B) Immunoblot of the CaV1.1 protein in TA muscles treated with ΔE29 or control morpholinos for the same 10 samples shown in (A). The CaV1.1-specific antibody detects a band around 200 kDa (arrow); * indicates non-specific cross-reactive bands. M, molecular weight marker. (C) Representative images of hematoxylin- and eosin-stained sections showing an increased fraction of fibers with central nuclei in TA muscles treated with ΔE29-morpholino (lower) compared with control morpholino (upper). (D) Quantification of the percentage of fibers with central nuclei in sections of contralateral TA muscles treated with control- (left) or ΔE29-skipping morpholinos (right) (P < 0.05, paired t-test).