Figure 4.

lrrk and rab7 genetically interact. (A–F) Lysotracker staining of follicle cells from stage 12 egg chambers. In wild-type follicle cells (A), most Lysotracker-positive vesicles are individual and distributed throughout the cytosol, although small clusters are often seen. Neither lrrk loss-of-function (B), nor overexpression of wild-type lrrk (C) significantly alters lysosome distribution. Expression of constitutively active rab7^Q67L (D) results in an increase in the formation of Lysotracker-positive clusters, and these tend to localize to the peri-nuclear region. lrrk loss-of-function significantly enhances lysosome clustering in follicle cells expressing rab7^Q67L (E), while overexpression of wild-type lrrk significantly reduces rab7^Q67L-induced lysosome clustering (F). Follicle cell nuclei are outlined with dashed gray lines in all Lysotracker images. (G) Quantification of lysosome clustering in the genotypes depicted in (A)–(F). Scale bars represent 5 μm.