Figure 6.
The GS mutation abrogates the ability of lrrk to inhibit rab7-induced lysosome clustering. (A–F) Lysotracker staining of follicle cells from stage 12 egg chambers of the indicated genotypes. Expression of constitutively active Rab7 (rab7Q67L, B) causes perinuclear clustering of Lysotracker-positive structures similar to that seen with expression of lrrkGS (D), while expression of rab7Q67L and lrrkGS together (E) causes a phenotype that is not significantly different from expression of lrrkGS alone. Expression of dominant-negative Rab7 (rab7T22N, C) causes lysosome dispersal. Expression of rab7T22N along with lrrkGS significantly reduces the lrrkGS-induced lysosome clustering. (G) Quantification of lysosome clustering in the indicated genotypes. Follicle cell nuclei are outlined with dashed gray lines in all Lysotracker images. Scale bars represent 5 μm.