Figure 7.
The GS mutation alters the characteristics of physical binding between Lrrk and Rab7 forms. (A) In lysates from cultured Drosophila S2 cells transfected with the depicted constructs, LrrkWT immunoprecipitates with both Rab7-GFP and Rab5-GFP, but not with GFP alone. The GS mutation reduces the physical interaction between Lrrk and Rab7, but does not affect binding to Rab5. (B) Whereas LrrkWT preferentially immunoprecipitates with the GTP-binding deficient dominant-negative version of Rab7, LrrkGS binds to Rab7DN and Rab7CA equally.