Table 1.
Absorption and fluorescence maxima of investigated dyes in dimethylsulphoxide (DMSO) solution, and emission maxima from stained frustules. Unsuccessful incorporation owing to death of the cells is indicated. M, methyl ester derivative; E, ethyl ester derivative.
| abs. (DMSO; nm) | emission (DMSO; nm) | cell status after incubation* | emission (frustule; nm) | |
|---|---|---|---|---|
| Rh 110 | 518 | 539 | vital | 535 |
| Rh 110-M (Rh 123) | 519 | 545 | vital | 544 |
| Rh 110-E | 519 | 548 | dead | — |
| Rh 19 | 535 | 561 | vital | 546 |
| Rh 19-M | 539 | 565 | dead | — |
| Rh 19-E (Rh 6G) | 539 | 564 | dead | — |
| Rh B | 564 | 592 | vital | 573 |
| Rh B-M | 566 | 593 | dead | — |
| Rh B-E | 566 | 593 | dead | — |
| Rh 101 | 580 | 606 | vital | 581, 622 |
| Rh 101-M | 588 | 615 | dead | — |
| Rh 101-E | 588 | 615 | dead | — |
*At a standard concentration of 1 µM dye and an incubation time of 3 days or longer.