Figure 1.

(A) Western blot of preS-pulldowned proteins; (B) Western blot of SCCA1-pulldowned proteins; (C) Western blot of FTL-pulldowned proteins. For A, MBP-preS was pre-incubated with either GST-FTL or GST before mixing with amylose beads; for B, MBP-SCCA1 was pre-incubated with either GST-FTL or GST before mixing with amylose beads; for C, HA-tagged preS and SCCA1 were coexpressed with His-tagged FTL protein in HepG2 cells before immunoprecipitation by anti-His-tag antibody.

Abbreviations: MBP, maltose binding protein; GST, glutathione-S-transferase; FTL, ferritin light chain; SCCA1, squamous cell carcinoma antigen 1.