Effect of overexpression of SCCA1, FTL on HBV attachment in HepG2 and CHO cells. Cells were transfected with pcDNA3.1 (column 1), pcDNA3.1- SCCA1 (column 2), pcDNA3.1-FTL (column 3), or both pcDNA3.1-SCCA1 and pcDNA3.1-FTL (column 4). HBsAg dissolved in RIPA buffer was measured by ELISA assay to quantify the HBV attachment experiment.
Abbreviations: CHO, Chinese hamster ovary; SCCA1, squamous cell carcinoma antigen 1; FTL, ferritin light chain; HBV, hepatitis B virus; HBsAg, hepatitis B virus surface protein antigens; RIPA, radio immunoprecipitation assay; ELISA, enzymelinked immunosorbent assay.