Skip to main content
. Author manuscript; available in PMC: 2012 Feb 22.
Published in final edited form as: Nat Cell Biol. 2010 Jan 17;12(2):185–192. doi: 10.1038/ncb2018

Figure 1. Injection of Mad2 L13Q or UbcH10C114S prevents anaphase onset in Drosophila syncytial embryos.

Figure 1

Embryos expressing His2A-mRFP1 were (a) not injected, (b) injected with 25 mg/ml of active Mad2 (Mad2L13Q) or (c) injected with 30 mg/ml of a dominant negative form of the human E2 ubiquitin-conjugating enzyme (UbcH10C114S); scale bars are 10 μm; bottom rows show higher magnifications (3x) of a single nuclear division. In all experiments performed (n≥25 embryos) both Mad2L13Q and UbcH10C114S induced a stable metaphase arrest.