Immunohistochemical staining of nestin in breast cancer. A,B) nestin-immunoreactivity was found in myoepithelium of the apparently normal mammary glands adiacently to tumoral mass (internal positive control); C), presence of cytoplasmic nestin expression in tumoral cells of a T4b breast cancer case with ER+, PgR+, HER2− phenotype. D), presence of nestin expression in tumoral cells of a T4d inflammatory breast cancer case with ER+, PgR−, HER2− phenotype; E), presence of nestin expression in tumoral cells of aT4d inflammatory breast cancer case with ER−, PgR−, HER2− triple-negative phenotype. F), intense nestin immunoreactivity in endothelial cells of peritumoral stroma. Original magnification: ×400 (A); ×1000 (B, D); ×630 (C, E, F).