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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Immunol. 2011 Aug 29;187(7):3738–3750. doi: 10.4049/jimmunol.1003783

Figure 9.

Figure 9

Enhanced immunogenicity when vaccines are given at separate sites is not due to presentation of antigens on separate APCs. BALB/c mice were primed with a mixture of AdHu5 vaccines as described in Table I. Eight weeks later they were boosted i.d. with 2 × 105 CD11c+ cells pulsed with either a mixture of the seven known PyMSP133 peptide epitopes and the two known PyCSP peptide epitopes, or pulsed separately with the PyMSP133 peptides and PyCSP peptides which were then mixed in a 1:1 ratio immediately prior to injection to give a total of 2 × 105 cells. Two weeks after immunization with peptide pulsed DCs, PBMCs were isolated and re-stimulated with peptides specific for PyMSP133 (A) and PyCSP (B) before ICS staining for IFN-γ.