Figure 3.

Dlx2 expression in the PVZ is restricted to the non-radial glial progenitor cells of the lateral wall. (A) Low magnification of a P1 mouse brain coronal section labeled with Pax6 (red) and Dlx2 (blue) antibodies showing an overall complementary, non-overlapping expression of these two genes in the PVZ. (B–E) High magnification of dorsally or laterally GFP electroporated cells labeled with Pax6 and Dlx2 antibodies at 6 h (B,C) and two days (D,E) after electroporation. Note that the dorsally electroporated cells expressed Pax6 but not Dlx2 (B,D). As expected the laterally electroporated cells did not express Pax6 at any time. The lateral radial glial neural stem cells did not express Dlx2 at 6 hpe (C), while the transit-amplifying and neuroblast progeny did express Dlx2 at 2 dpe (E). Arrowheads: GFP⁺/marker⁺ cells, arrows: GFP⁺/marker⁻ cells. Scale bar: 10 μm.