Figure 3 .

Presence or absence of Osiris family members in various species. Gray indicates presence. Diptera and holometabolous insect-specific presence is shown in red and orange colors, respectively. An asterisk indicates that the gene is inverted relative to Osiris 2 in each species, and a left arrow indicates that the region including more than one gene is inverted. A question mark indicates when the relative gene direction cannot be inferred because of separated contigs. A number indicates the number of duplicated copies. One non-Osiris gene, NPFR1, is also included in the figure because it is tightly linked within the Osiris gene cluster. In the D. melanogaster genome, genes from Osiris 1 to Osiris 20 are located within a tightly linked 167.5-kb region on the chromosomal arm 3R (83D-E). Although Osiris 22 and Osiris 23 are also on the 3R but located distantly (87E and 99F, respectively), Osiris 21 is located on the chromosomal arm 2L (32E). In some insects, Osiris 23 is located on a different chromosome and they are indicated by double-line boxes. See Table S3 for detailed information of all genes we identified.